Identification of a novel variant of hepatitis E virus in Italy

Hepatitis E infection is typically associated with areas in which hepatitis E virus (HEV) is endemic. Except for a few cases in Europe and in the Unit ed States, acute hepatitis E is usually associated with travel to endemic a reas. We set out to determine the etiologic role of HEV in acute non-A-C he patitis in Italy. The presence of HEV-RNA and antibody was determined in 21 8 patients diagnosed with acute viral non-A-C hepatitis. Acute hepatitis E infection was defined by the presence of HEV-RNA in sera and positivity for IgM anti-HEV and seroconversion to IgG anti-HEV. Acute hepatitis E was fou nd in 10.1% of the patients with acute non-A-C, with 95.5% exhibiting a ben ign course. A more severe course was observed in a patient co-infected with HAV and HEV. Most cases were travelers to endemic areas, although 18.2% re ported no travel. One patient was from a household with an infected patient . Sequence analyses of the polymerase chain reaction (PCR) product derived from a patient who never visited endemic areas, identified an isolate that is divergent significantly from all reported isolates of HEV (79.5-85.8% nu cleotide identity). Evidence from this study suggests that HEV accounts for approximately 10% of acute non-A-C viral hepatitis in Italy, diagnosed gen erally in travelers returning from endemic areas. However, the identificati on of a new HEV variant in an individual who never indicated travel or cont act with individuals associated with endemic areas, suggests that this viru s may be native to (C) 1999 Wiley-Liss, Inc.