J. Vioque et al., The malate dehydrogenase gene from Botryococcus braunii (Chlorophyta, Chlorophyceae): Cloning, sequence analysis, and expression in Escherichia coli, J PHYCOLOGY, 35(1), 1999, pp. 121-127
The gene for malate dehydrogenase from Botryococcus braunii Kutzing (Chloro
phyceae) was isolated, The gene codes for a protein of 316 amino acids with
a calculated molecular weight of 32.9 kDa, In the N-terminal region, a pyr
idine nucleotide-binding domain was observed, The G+C content of the gene i
s 65%, Six copies of the (A/G)GGCGG hexanucleotide, which forms the core bi
nding site for the cellular transcription factor Sp1, were observed in the
promoter region, Southern blot analysis showed that malate dehydrogenase is
encoded by a single gene in B. braunii, Escherichia coli cells in which th
e B, braunii gene was expressed showed 3.6 times more activity in the conve
rsion of oxaloacetate to malate than the noninduced ones, confirming the id
entity of the gene, Botryococcus braunii malate dehydrogenase has been subj
ected to phylogenetic analyses by the neighbor-joining and parsimony proced
ures.