Sr2+-dependent asynchronous evoked transmission at rat striatal inhibitorysynapses in vitro

1. At striatal inhibitory synapses in cell culture, replacement of extracel lular Ca2+ with Sr2+ desynchronized inhibitory postynaptic currents (IPSCs) , reducing their peak amplitude and producing a succession of late, asynchr onous synaptic events (late release). In the averaged IPSC waveform this re sulted in an increase in both the fast and the slow decay time constant as well as in the time to peak. 2. Rapid removal of extracellular Sr2+ during late release was without effe ct on the time course of the averaged IPSC. Thus, late release is not depen dent on continuous Sr2+ influx, but must be related to the way in which Sr2 +, as opposed to Ca2+, interacts with constituents of the intracellular spa ce. 3. After application of the membrane-permeant acetoxymethyl ester (AM) form of the Ca2+ chelator BAPTA, Sr2+-induced late release was greatly reduced and the kinetics of the Sr2+ dependent IPSC approached those of the Ca(2+-) dependent response. EGTA AM had a similar but less pronounced effect. 4. Using rapid solution exchange, we stimulated synapses first in Sr2+- or Ca2+ - and 100-300 ms afterwards in Ca2+-containing solution. Paired-pulse facilitation of late release was the same whether the conditioning pulse in duced a presynaptic influx of Sr2+ or of Ca2+. 5. It is concluded that Sr2+-mediated asynchrony is probably due to a less efficient intraterminal buffering of Sr2+ as opposed to Ca2+, allowing for Sr2+ ions to activate release in an area less confined to the immediate vic inity of the presynaptic Ca2+ channel. This hypothesis explains both the ac tion of endogenous buffers and the apparent lack of specific facilitatory i nteraction between Ca2+-mediated and Sr2+-induced late release.