Chemotaxonomy of triterpenoid pattern of HPLC of Ganoderma lucidum and Ganoderma tsugae

Three strains of Ganoderma tsugae (CCRC36065, CCRC37034, CCRC37038) and thr ee strains of Ganoderma lucidum (CCRC36021, CCRC37029, CCRC37033) were cult ivated. Their triterpenoid patterns of the fruit body were analyzed by reve rse phase HPLC using a gradient elution of acetonitrile/2% acetic acid (1/4 and 1/2). The triterpenoid patterns of G. tsugae and G. lucidum are differ ent. But similar 2 and 3 dimensional patterns are obtained among three stra ins of G. tsugae: Different patterns are found among different strains of G . lucidum. Ganoderic acid A(1), B(2), C(3) and D(4) were isolated from the ethanol extract of G. tsugae.