Changes in hyaluronidase, acrosin, and N-acetylhexosaminidase activities of dog sperm after incubation

Hyaluronidase, acrosin and N-acetylhexosaminidase activities were examined in sperm collected from 12 beagle dogs and in culture medium after 0.5 hr a nd 7 hr of sperm incubation. The activities of the three enzymes were signi ficantly higher at 7 hr than at 0.5 hr (P<0.05, 0.01), and the increases we re associated with sperm capacitation. It was considered that the three enz ymes in the dog sperm are related to fertilization by reason of the finding s of the release of these enzymes from the sperm into the medium after 7 hr of incubation.