A flow-injection system for iodide determination has been developed based o
n the Sandell and Kolthoff reaction in which iodide catalyses the reaction
of Ce(IV) and As(III). The decrease in Ce(IV) concentration measured colori
metrically in the range 410-430 nm, after a fixed time interval, is thus de
pendent on the iodide concentration. The method is simple and sensitive to
very low concentrations of iodide. The detection limit is 0.8 mu g I- L-1 (
S/N = 3). The calibration graph was found to be linear over a range of 0-7
mu g L-1. The effects of some interferents, including halides and others th
at are found in urine and milk samples (glucose, ascorbic acid, thiocyanate
ion, bicarbonate ion and calcium ion), were studied. The presence of fluor
ide ion, thiocyanate ion, or oxidizable organic molecules caused serious po
sitive errors. Sulfate ion was the only interferent examined that caused a
negative error, but the effect of sulfate can be ignored when its concentra
tion is less than 10(5) mg L-1. (C) 1999 John Wiley & Sons, Inc.