ANALYSIS OF THE LARGE (L) PROTEIN GENE OF THE PORCINE RUBULAVIRUS LPMV - IDENTIFICATION OF POSSIBLE FUNCTIONAL DOMAINS

The complete nucleotide sequence of the porcine rubulavirus LPMV (La P iedad Michoacan virus) large (L) protein gene was determined and analy sed. The L mRNA was found to span 6786 nucleotides, containing one sin gle large open reading frame (ORF), putatively encoding a polypeptide of 2251 amino acids. By aligning the amino acid sequence of the LPMV L -protein with L-proteins of a number of viruses belonging to the order mononegavirale, a high degree of similarity between the LPMV L-protei n and other rubula virus L-proteins was demonstrated, extending throug h almost the whole protein. Additionally we could identify several reg ions as being highly conserved among all studied viruses of the order mononegavirale. The significance of these regions are discussed. (C) 1 997 Elsevier Science B.V.