Binding of polylysine to protein kinase CK2, measured by surface plasmon resonance

The interaction between protein kinase CK2 and polylysine has been studied by Surface Plasmon Resonance (SPR). The binding process has a very low ener gy of activation, it is irreversible, and too slow as to explain the enzyme activity stimulation as a direct consequence of the polylysine binding. Th e polylysine interaction with a peptide substrate and with casein are faste r, and in agreement with a substrate-mediated mechanism of activity stimula tion. After several hours of incubation, the binding of polylysine to CK2 p roduces the loss of enzymatic activity.