Searching interaction partners of protein kinase CK2 beta subunit by two-hybrid screening

Authors
Grein, S Raymond, K Cochet, C Pyerin, W Chambaz, EM Filhol, O
Citation
S. Grein et al., Searching interaction partners of protein kinase CK2 beta subunit by two-hybrid screening, MOL C BIOCH, 191(1-2), 1999, pp. 105-109
Citations number
23
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR AND CELLULAR BIOCHEMISTRY
ISSN journal
03008177 → ACNP
Volume
191
Issue
1-2
Year of publication
1999
Pages
105 - 109
Database
ISI
SICI code
0300-8177(199901)191:1-2<105:SIPOPK>2.0.ZU;2-A
Abstract
To date, the intracellular regulation of protein kinase CK2 is unknown. How ever it was observed that the enzyme associates with several intracellular proteins and the formation of such molecular complexes may represent a mech anism for the control of CK2 activity. Using the Interaction Trap system in yeast, with the CK2 beta as a bait, we looked for CK2 partners. We present the identification of new potential partners of CK2 beta and it is hoped t hat their classification will help in understanding the physiological roles and the regulation of CK2 in the cell.