DNA-protein interactions in the CCAAT box region of the murine lactate dehydrogenase C promoter

Electrophoretic Mobility (EMSA), using oligonucleotides containing CCAAT bo x sequences from the murine ldhc promoter show the presence of CCAAT bindin g proteins in nuclear extracts from liver and testis, In a liver extract, a single shifted band is seen. However, in the testis extract, two shifts ar e observed, one of which may be due to a testis specific isoform of CCAAT b inding factor (CBF). Southwestern analysis with an oligonucleotide probe co ntaining these sequences reveals the presence of a protein of approximately 120 kD in the testis extract. In the liver extract, a 70-kD protein binds the probe. An antibody against HeLa CBF causes a supershift in testis nucle ar extract. (C) 1999 Wiley-Liss, Inc.