Si. Kwon et Cs. An, Isolation and characterization of the catalase gene from Rhizobium sp. SNU003, a root nodule symbiont of Canavalia lineata, MOL CELLS, 9(1), 1999, pp. 49-55
A catalase gene from Rhizobium sp. SNU003, a root nodule symbiont of Canava
lia lineata, was cloned and its nucleotide sequence was determined. The Rhi
zobium DNA of about 280 bp was amplified using two PCR primers synthesized
from the conserved sequences of the type I catalase gene. The nucleotide se
quence of the amplified fragment revealed three regions that were conserved
in the catalase, showing it as being part of the catalase gene. A genomic
Southern hybridization using this fragment as a probe showed that the 5.5 k
b PstI, 1.8kb EcoRI, and 0.7 kb StyI fragments hybridized strongly with the
probe. The Rhizobium genomic Library constructed into the EMBL3 vector was
screened, and one catalase clone was selected, The nucleotide sequence of
the 5.5 kb PstI fragment from the clone revealed an open reading frame of 1
455 bp, encoding a polypeptide of 485 amino acids with a molecular mass of
54,958 Da and a pi of 6.54, The predicted amino acid sequence of the catala
se is 66.3% identical to that of Bacteroides fragilis, but was only 53.3% i
dentical to the Rhizobium meliloti catalase.