Lymph node revealing solution: A rapid method for the fixation of cystectomy specimens

The objective of this study was to describe the use of the lymph node revea ling solution (LNRS) for rapid fixation of total cystectomy specimens, and to compare it with formalin fixation. LNRS is a mixture of 95% ethanol, diethyl ether, glacial acetic acid and bu ffered formalin (65:20:5:10 v/v) prepared under a fume-hood. Sixteen consec utive cystectomy specimens were fixed for two hours either in LNRS or in bu ffered formalin. Representative sections were embedded in paraffin, section ed and stained with H&E, periodic acid Schiff, alcian-blue, and immunostain ed for cytokeratins 20, high and low molecular weight cytokeratins, prostat ic specific antigen, Factor VIII related antigen, s-100 protein, and protei n kinase C isoenzymes. Results showed that the tissues were well fixed after 2 hours in LNRS, and were not fixed after 2 hours in formalin. Processing and sectioning of the paraffin blocks of the LNRS fixed tissue was excellent; it was impossible i n the sections fixed for 2 hours in formalin. All the stains were excellent after LNRS fixation. We conclude that fixation of cystectomy specimens in LNRS requires only two hours and results in excellent stained slides. It is therefore recommended for cystectomy specimens.