Ja. Ozga et Dm. Reinecke, Interaction of 4-chloroindole-3-acetic acid and gibberellins in early pea fruit development, PLANT GR R, 27(1), 1999, pp. 33-38
In pea, normal pod (pericarp) growth requires the presence of seeds; and in
the absence of seeds, gibberellins (GAs) and/or auxins can stimulate peric
arp growth. To further characterize the function of naturally occurring pea
GAs and the auxin, 4-chloroindole-3-acetic acid (4-Cl-IAA), on pea fruit d
evelopment, profiles of the biological activities of GA(3), GA(1), and 4-Cl
-IAA on pericarp growth were determined separately and in combination on po
llinated deseeded ovaries (split-pericarp assay) and nonpollinated ovaries.
Nonpollinated ovaries (pericarps) responded differently to exogenous GAs a
nd 4-Cl-IAA than pollinated deseeded pericarps. In nonpollinated pericarps,
both GA(3) and 4-Cl-IAA stimulated pericarp growth, but GA3 was significan
tly more active in stimulating all measured parameters of pericarp growth t
han 4-Cl-IAA. 4-Cl-IAA, GA(1), and GA(3) were observed to stimulate pericar
p growth similarly in pollinated deseeded pericarps. In addition, the syner
gistic effect of simultaneous application of 4-Cl-IAA and GAs on pollinated
deseeded pericarp growth supports the hypothesis that GAs and 4-Cl-IAA are
involved in the growth and development of pollinated ovaries.