Genetic and physical characterization of a region of Arabidopsis chromosome 1 containing the CLAVATA1 gene

With the advance of Arabidopsis as a model system for understanding plant g enetics, development and biochemistry, a detailed description of the genome is necessary. As such, focused projects are underway to map and sequence t he Arabidopsis nuclear genome. We have characterized a region of chromosome 1, surrounding the CLAVATA1 (CLV1) locus. Three (RFLP) clones were mapped relative to clv1-1, and were used to construct an ca. 700 kb yeast artifici al chromosome (YAC) contig. Three cosmids spanning the CLV1 locus were anal yzed and ca. 24 kb of genomic DNA was sequenced, including a continuous str etch of 18 kb. In addition to generating clones in this region of chromosom e 1, we have analyzed the size, spacing and organization of several contigu ous genes.