The use of liquid phase carriers for large scale oligonucleotide synthesisin solution via phosphoamidite chemistry

Nucleoside derivatives coupled to a multifunctional highly symmetric primar y amine 3 built the fundamental of a convenient method for large scale olig onucleotide synthesis in solution. The basic purification for the fast isol ation of intermediates is obtained by gel permeation chromatography. Monome r and dimer phosphoamidites (1a-d; 2a,b) are used for the preparation of sh ort oligodeoxynucleotides. Total cycle yields between 81 and 95 % and avera ge cycle yields of 87 % were obtained. MALDI-TOF-mass spectrometry was used for the analysis of the fully protected intermediates during synthesis. (C ) 1999 Elsevier Science Ltd. All rights reserved.