Altered product pattern of a squalene-hopene cyclase by mutagenesis of active site residues

Authors
Merkofer, T Pale-Grosdemange, C Wendt, KU Rohmer, M Poralla, K
Citation
T. Merkofer et al., Altered product pattern of a squalene-hopene cyclase by mutagenesis of active site residues, TETRAHEDR L, 40(11), 1999, pp. 2121-2124
Citations number
8
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
40
Issue
11
Year of publication
1999
Pages
2121 - 2124
Database
ISI
SICI code
0040-4039(19990312)40:11<2121:APPOAS>2.0.ZU;2-D
Abstract
Amino acid residues lining the catalytic cavity of squalene-hopene cyclase of Alicyclobacillus acidocaldarius have been mutated. Alterations of His451 to Ala and Trp489 to Ala resulted in reduced enzymatic activity, while the product patterns were identical to that of the wild-type. Mutation of Phe6 01 to Ala led to the enhanced formation of a tetracyclic triterpene, 17-iso dammara-20(21),24-diene 4, and of Tyr420 to Ala to a significant alteration of the product pattern. (C) 1999 Published by Elsevier Science Ltd. All ri ghts reserved.