Competitive immunoassay (Cat-EIA), a helpful technique for catalytic antibody detection. Part I.

Authors
Taran, F Renard, PY Creminon, C Valleix, A Frobert, Y Pradelles, P Grassi, J Mioskowski, C
Citation
F. Taran et al., Competitive immunoassay (Cat-EIA), a helpful technique for catalytic antibody detection. Part I., TETRAHEDR L, 40(10), 1999, pp. 1887-1890
Citations number
14
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON LETTERS
ISSN journal
00404039 → ACNP
Volume
40
Issue
10
Year of publication
1999
Pages
1887 - 1890
Database
ISI
SICI code
0040-4039(19990305)40:10<1887:CI(AHT>2.0.ZU;2-K
Abstract
A competitive immunoassay procedure for the screening of catalytic antibodi es is reported. This screening approach (Cat EIA) is a modification of the well-known Cat-ELISA technique avoiding the substrate modification step. It has been developed for a bimolecular reaction and has been tested on a hig h number of hybridoma clones. The current study explores for the first time the utility and feasibility of this method for the early detection of cata lytic antibodies. (C) 1999 Elsevier Science Ltd. All rights reserved.