Cerebrospinal fluid (CSF) evaluation can yield important information in beh
avioral, pharmacologic, and toxic assessment of the nervous system. Recentl
y identified neurotransmitters are available and quantifiable in CSF: CSF c
ollection in macaques is easy to perform and extremely safe for the monkeys
. This paper outlines a simple, harmless, and reliable method to collect CS
F from the atlanto-occipital cistern of macaques.