A HIGH-DENSITY SOYBEAN GENETIC-MAP BASED ON AFLP MARKERS

Genetic marker systems have improved dramatically in the past 10 yr, E ach new system needs to be evaluated for its distribution of markers o n genetic linkage maps to validate its use for genetic analysis. The r esulting maps are also useful for establishing the genetic positions o f genes affecting important phenotypes. We have constructed a high den sity map in soybean [Glycine mar (L.) Merr.] using a 300 RIL (recombin ant inbred line) population from BSR-101 x PI437,654 by first construc ting an RFLP (restriction fragment length polymorphism) ''scaffold'' m ap based on the entire population, The RFLP anchored map was then furt her populated with AFLP (amplified fragment length polymorphism) marke rs based on only a 42 RIL subset, We report here an 840 marker map con sisting of 165 RFLP, 25 RAPD (random amplified polymorphic DNA), and 6 50 AFLP markers spread over 28 linkage groups representing 3441 cM dis tance, Although clustering of AFLP markers occurred, markers were mapp ed to every linkage group and were well distributed relative to other marker systems, The AFLP marker system appears to be a useful approach for generating high density genetic maps in soybean.