Zp. Wang et al., Basic fibroblast growth factor enhanced LAK cell cytotoxicities against human bladder neoplasm cells, ACT PHAR SI, 20(3), 1999, pp. 276-278
AIM: To study the effects of basic fibrolast growth factor (bFGF) on the pr
oliferation of lymphokine-activated killer (LAK) cells fi om patients with
bladder cancer and LAK cells cytolysis against bladder tumor cells. METHODS
: LAK cell proliferation was assayed in the presence of various concentrati
ons of bFGF combined with interleukin-2 (IL-2) by cell count. Cytotoxicity
of LAK cells against bladder cancer cell line EJ cells and bladder tumor ce
lls (BTC) from patients was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide (MTT) assays. RESULTS: The proliferation of per
ipheral blood monocytes (PBMC) was inhibited by bFGF 5 mu g. L-1. bFGF did
not affect the stimulation of LAK cells induced by IL 2. The LAK cell numbe
rs in the combination of IL-2 with bFGF were not significantly different co
mpared with that treated with IL-2 alone. bFGF enhanced cytotoxicity of LAK
cells against bladder cancer cell line EJ cells or ETC, respectively. CONC
LUSION: Although the proliferation of PBMC was inhibited by bFGF, bFGF incr
eased LAK cell cytotoxicity against bladder neoplasm cells.