Basic fibroblast growth factor enhanced LAK cell cytotoxicities against human bladder neoplasm cells

Citation
Zp. Wang et al., Basic fibroblast growth factor enhanced LAK cell cytotoxicities against human bladder neoplasm cells, ACT PHAR SI, 20(3), 1999, pp. 276-278
Citations number
6
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
02539756 → ACNP
Volume
20
Issue
3
Year of publication
1999
Pages
276 - 278
Database
ISI
SICI code
0253-9756(199903)20:3<276:BFGFEL>2.0.ZU;2-I
Abstract
AIM: To study the effects of basic fibrolast growth factor (bFGF) on the pr oliferation of lymphokine-activated killer (LAK) cells fi om patients with bladder cancer and LAK cells cytolysis against bladder tumor cells. METHODS : LAK cell proliferation was assayed in the presence of various concentrati ons of bFGF combined with interleukin-2 (IL-2) by cell count. Cytotoxicity of LAK cells against bladder cancer cell line EJ cells and bladder tumor ce lls (BTC) from patients was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assays. RESULTS: The proliferation of per ipheral blood monocytes (PBMC) was inhibited by bFGF 5 mu g. L-1. bFGF did not affect the stimulation of LAK cells induced by IL 2. The LAK cell numbe rs in the combination of IL-2 with bFGF were not significantly different co mpared with that treated with IL-2 alone. bFGF enhanced cytotoxicity of LAK cells against bladder cancer cell line EJ cells or ETC, respectively. CONC LUSION: Although the proliferation of PBMC was inhibited by bFGF, bFGF incr eased LAK cell cytotoxicity against bladder neoplasm cells.