Jy. Honda et al., Isoelectric focusing electrophoresis and RFLP analysis: Two methods for immature Orius spp. identification, APPL ENT ZO, 34(1), 1999, pp. 69-74
Two techniques were used to discriminate between second instar Orius minutu
s (L) and Orius sauteri (Poppius) which occur sympatrically in eggplant fie
lds of Japan. Isoelectric focusing (IEF) was used to discriminate between t
he two species utilizing the enzyme isocitrate dehydrogenase (IDH). Moreove
r, IEF was also shown to detect the presence of the prey Thrips palmi Karny
in O. sauteri nymphs immediately after feeding by using phosphogluco-isome
rase (PGI). PCR generated internal transcribed spacer 1 (ITS-1) regions for
nymphs of both species were also diagnostic as the O. minutus PCR product
was much larger than the O. sauteri PCR product (900 vs. 600 bp). Moreover,
by comparing ITS-1 PCR product sizes and using the restriction enzyme Hinf
I on ITS-I products of similar size to produce restriction fragment length
polymorphisms (RFLPs), consistent differences among five Japanese Orius spe
cies were also demonstrated.