Differentiation of sturgeon caviar by single strand conformation polymorphism (PCR-SSCP) analysis

Authors
Rehbein, H Gonzales-Sotelo, C Perez-Martin, R Quinteiro, J Rey-Mendez, M Pryde, S Mackie, IM Santos, AT
Citation
H. Rehbein et al., Differentiation of sturgeon caviar by single strand conformation polymorphism (PCR-SSCP) analysis, ARCH LEBENS, 50(1), 1999, pp. 13-17
Citations number
14
Categorie Soggetti
Food Science/Nutrition
Journal title
Archiv für Lebensmittelhygiene
ISSN journal
0003925X → ACNP
Volume
50
Issue
1
Year of publication
1999
Pages
13 - 17
Database
ISI
SICI code
0003-925X(199901/02)50:1<13:DOSCBS>2.0.ZU;2-L
Abstract
Two fragments of the mitochondrial cytochrome b gene were analysed by PCR-S SCP to differentiate sturgeon caviar. Amplicons of 116, respectively 123, n ucleotides were transformed into single strand DNA, separated by native pol yacrylamide gel electrophoresis, and visualised by silver staining. Several combinations of gel and buffer types were tested. Species specific patterns of single strand DNA were obtained for beluga-, o sietra-, and sevruga-caviar.