Cloning of a GADD34-like gene that interacts with the zinc-finger transcription factor which binds to the p21(WAF) promoter

A histone deacetylase inhibitor has been shown to induce differentiation of many cancer cells and senescence-like state of human fibroblasts. Previous ly, our data suggested that the region responsive to trichostatin A (TSA), a specific inhibitor of histone deacetylase, treatment in the p21(WAF1) pro moter is located -100 bp upstream from transcription initiation site and co ntains a GC-box where both Sp1 and Sp3 are responsible. Here we show that a nother zinc-finger transcription factor, BFCOL1, which binds to the proxima l pro alpha 2(I) collagen promoter, could also bind to this GC-box of the p 21 promoter. In addition, we cloned a gene whose product interacts with thi s factor by yeast two-hybrid method. The cloned gene was a variant of GADD3 4 and lacking one PEST region. We found that this cDNA product decreased th e DNA binding activity of BFCOL1 to the GC-rich region of p21 minimal promo ter. (C) 1999 Academic Press.