Novel substrates of yeast alcohol dehydrogenase - 4. Allyl alcohol and ethylene glycol

Authors
Trivic, S Leskovac, V
Citation
S. Trivic et V. Leskovac, Novel substrates of yeast alcohol dehydrogenase - 4. Allyl alcohol and ethylene glycol, BIOC MOL B, 47(1), 1999, pp. 1-8
Citations number
11
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
ISSN journal
10399712 → ACNP
Volume
47
Issue
1
Year of publication
1999
Pages
1 - 8
Database
ISI
SICI code
1039-9712(199901)47:1<1:NSOYAD>2.0.ZU;2-W
Abstract
In the present work, we have determined the steady-state kinetic constants for yeast alcohol dehydrogenase-catalyzed oxidation of allyl alcohol (H2C=C H . CH2OH) and ethylene glycol (HOCH2. CH2OH) with NAD(+), at pH 8.0; also, a kinetic mechanism for the former reaction was determined at the same pH. In addition, it was found that acrolein is a potent inhibitor of yeast alc ohol dehydrogenase.