S. Porebski et Pm. Catling, RAPD analysis of the relationship of North and South American subspecies of Fragaria chiloensis, CAN J BOTAN, 76(10), 1998, pp. 1812-1817
Citations number
31
Categorie Soggetti
Plant Sciences
Journal title
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
To improve the intraspecific classification of Fragaria chiloensis (L.) Duc
hesne, 35 plants including 5 North American ssp. lucida, 15 North American
ssp. pacifica, and 15 South American ssp. chiloensis were analysed using ra
ndom amplified polymorphic DNA (RAPDs). From 100 primers screened, 12 were
selected providing 62 scorable polymorphic bands. The phenogram (cophenetic
correlation, r = 0.99) based on UPGMA clustering of Jaccard's coefficients
revealed a clear division between North American and South American plants
, but only partial separation was shown between the two North American subs
pecies. This is the first comprehensive molecular evidence for major geneti
c divergence between the North American and South American subspecies of F:
chiloensis and suggests greater genetic variation within the Canadian mate
rial of the North American ssp. pacifica than within the South American ssp
, chiloensis. These findings strongly support protection and utilization of
wild Canadian Fragaria germplasm for crop improvement.