Inhibition of benzo[a]pyrene-induced mutagenesis by (-)-epigallocatechin gallate in the lung of rpsL transgenic mice

Epigallocatechin gallate (EGCG) is a major water-soluble component of green tea. The antimutagenic activity of EGCG against benzo[a]pyrene (B[a]P)-ind uced mutations was assessed by using transgenic mice carrying the rpsL gene as a monitor of mutations. Seven-week-old male mice were given drinking wa ter containing EGCG for 3 weeks, On day 7, mice were treated with a single i.p. injection of B[a]P (500 mg/kg body wt). Two weeks after the injection, the mutations in the rpsL gene were analyzed. B[a]P treatment resulted in an similar to 4-fold increase of mutation frequency at the rpsL gene in the lung. An similar to 60% reduction in the B[a]P-induced mutations in the lu ng was observed when mice were given EGCG at concentrations >0.005%, B[a]P- induced mutations mainly occurred at G:C basepairs in the several specific nucleotide sequences of the rpsL gene. These were AGG, CGG, CGT, TGG, TGC a nd GGT: all of them contained a guanine residue. Mutations seen similarly i n the human Ki-ms codon 12 or p53 codons 157, 248, and 273 of lung tumor we re also found in the rpsL gene, and the mutations were suppressed by the EG CG treatment. In conclusion, the antimutagenic effects of EGCG for B[a]P-in duced mutagenesis in vivo suggest that drinking green tea may reduce the tu mor-initiating potency of B[a]P in the lung.