Determination of hainanmycin in the aqueous-alcohol solution by Fourier transform infrared spectroscopy

Fourier transform infrared(FT-IR) spectroscopy equipped with horizontal att enuated total reflection(ATR) accessory was applied to the multi-component quantitative analysis in organic phase, A novel method was developed to dir ectly determine the Hainanmycin (HNMS, an insoluble antibiotic from the fer mentation) in water and ethyl alcohol solution. Calibration models were est ablished on the basis of partial least squares with infrared spectra (IR) a nd second-derivative absorbance spectra(DIR) of the standard mixtures. The better predicted results is obtained especially at lower concentration of H NMS by DIR method. The standard errors of prediction of HNMS by IR or DIR a re 0.18 and 0.19 g/L, respectively, The standard errors of prediction of wa ter and alcohol are 0.001 1 volume ratio by either quantitative method. The method is rapid and convenient without any pre-treatment.