H. Thorlacius et al., CYTOKINE-INDUCED LEUKOCYTE ROLLING IN MOUSE CREMASTER MUSCLE ARTERIOLES IS P-SELECTIN DEPENDENT, American journal of physiology. Heart and circulatory physiology, 41(4), 1997, pp. 1725-1729
Leukocyte rolling and adhesion are generally observed in venules but r
arely observed in arterioles. With the use of intravital microscopy, w
e found that a 4-h treatment with interleukin-1 beta (IL-1 beta) and t
umor necrosis factor-alpha (TNF-alpha) dose dependently induced leukoc
yte rolling and adhesion in arterioles of the mouse cremaster muscle.
The rolling response lasted more than 24 h and was completely inhibite
d by treatment with the sulfated polysaccharide fucoidin. Moreover, we
found that costimulation with IL-1 beta and TNF-alpha for 4 h synergi
stically increased arteriolar leukocyte rolling, i.e., threshold doses
of IL-1 beta and TNF-alpha together caused a more than 10-fold increa
se of rolling in arterioles compared with the sum of the individual re
sponses. This rolling interaction was abolished by treatment with a mo
noclonal antibody directed against P-selectin (RB40.34), but it appare
ntly was unaffected by a monoclonal antibody against L-selectin (MEL-1
4). Taken together, our functional data show that IL-1 beta and TNF-al
pha separately induce and synergistically increase P-selectin-dependen
t leukocyte rolling and firm adhesion in mouse cremaster arterioles.