Establishing the diets of ancient human populations is an integral componen
t of most archaeological studies. Stable isotope analysis of well-preserved
bone collagen is the most direct approach for a general assessment of pale
odiet, However, this method has been limited by the scarcity of well-preser
ved skeletal materials for this type of destructive analysis. Hair is prese
rved in many burials, but is often overlooked as an alternative material fo
r isotopic analysis. Here we report that the stable carbon and nitrogen iso
tope values for the hair of the 5200 year-old Ice Man indicates a primarily
vegetarian diet, in agreement with his dental wear pattern. Whereas previo
us investigations have focused on bone collagen, the stable isotope composi
tion of hair may prove to be a more reliable proxy for paleodiet reconstruc
tion, particularly when skeletal remains are not well preserved and additio
nal archaeological artifacts are unavailable.