H. Enroth et al., In vitro aging of Helicobacter pylori: Changes in morphology, intracellular composition and surface properties, HELICOBACT, 4(1), 1999, pp. 7-16
Background. During the conversion from the bacillary into the coccoid form,
Helicobacter pylori organisms are known to change extensively. The aim of
this study was to determine some of the changes that occur regarding morpho
logy, intracellular composition and surface properties during the aging of
bacteria in vitro.
Materials and Methods. H. pylori from agar plate cultures of different ages
was used in this study. The intracellular composition of the two morpholog
ical forms of the bacteria was tested by density centrifugation, DNA extrac
tion and quantitative OD, mRNA and ATP measurements. Immunoblotting was use
d to observe changes in secreted/superficial protein patterns, and hydropho
bicity measurements were used to observe changes in surface properties.
Results. All bacillary H. pylori organisms changed morphology gradually ove
r 10 days of culture. Rods had a higher density than cocci; bacteria stored
in PBS had the highest density and bacteria stored in water had the lowest
. The quantitative DNA, RNA and ATP content were reduced in the aging bacte
ria. Fewer immunogenic proteins were expressed, and an increased surface hy
drophobicity was observed in the older cultures.
Conclusion. This study highlights several aspects of H. pylori aging in vit
ro and shows some of the differences that exist between bacillary and cocco
id forms. This information is important for understanding the transmission
and survival of H. pylori outside the human host, as the degradative change
s in the intracellular composition and the surface properties shown here po
int to dead bacteria, and not to a viable but nonculturable form.