A. Kondo et al., Establishment and cellular characteristics of a hepatocyte cell line (OUMS-31) derived from an acatalasemic mouse, IN VITRO-AN, 35(3), 1999, pp. 155-158
Liver cell lines with very low catalase activity were established from an a
catalasemic mouse. Hepatocytes isolated by a collagenase-liver-perfusion te
chnique were cultured in Williams' E medium supplemented with 10% fetal bov
ine serum. The acatalasemic liver cell line showed approximately 20% of the
catalase activity of a normal mouse liver cell line, whereas its glutathio
ne peroxidase activity was approximately equal to that of the normal liver
cell line. DNA sequence analysis of this cell line showed the same mutation
in the catalase gene as is seen in the acatalasemic mouse. Our observation
of intracellular content of hydrogen peroxide (H2O2) radical and increased
susceptibility of the cells to H2O2 were compatible with the existence of
low catalase activity in the acatalasemic mouse. This hepatocyte cell line
should be useful for studying effects of oxidative radical stress at the ce
llular level.