A. Antipenko et al., Effect of chronic electrostimulation of rabbit skeletal muscle on calmodulin level and protein kinase activity, INT J BIO C, 31(2), 1999, pp. 303-310
Citations number
36
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(a) Chronic electrostimulation of fast-twitch skeletal muscles makes them r
esemble slow-twitch muscles. The involvement of second-messenger cascades i
n this muscle reprogramming is not well understood. The goal of this study
was to examine protein kinase activities and calmodulin levels as a functio
n of the duration of electrostimulation,
(b) Fast-twitch rabbit muscle was subjected to continuous low-frequency ele
ctrostimulation for 2 weeks, The extensor digitorum longus was taken and ex
amined for calmodulin concentration and cAMP-dependent (PKA), Ca2+-phosphol
ipid-dependent (PKC) and Ca2+-calmodulin-dependent (CaM kinase or PKB) prot
ein kinase activities.
(c) Electrostimulation for 14 days led to a significant increase in total c
almodulin level and PKB activity, both rising in the cytosolic fraction. Pr
otein kinase C translocated to the membrane fraction, although total activi
ty did not change.
(d) These changes could be related with electrostimulation-induced changes
in excitation-contraction coupling. (C) 1999 Elsevier Science Ltd. All righ
ts reserved.