DEVELOPMENT OF A PROTOCOL FOR THE QUANTITATIVE-EVALUATION OF CONTRASTIN NMR IMAGES OF CRYOPROTECTIVE SOLVENTS IN INTACT TISSUES

In a recent study (Fyfe et al., Magn. Res. Chem. 32, 1994), chemical s hift selective nuclear magnetic resonance (NMR) imaging was used to mo nitor the location and concentration of water and Me2SO in test sample s. This study verifies that the technique can be applied to quantifica tion of Me2SO in rat kidney and liver at temperatures commonly used fo r introducing cryoprotectants into tissues. A brief practical guide is provided for cryobiologists interested in using NMR imaging for such studies. (C) 1997 Academic Press.