Exposure of thawed frozen bull sperm to a synthetic peptide before artificial insemination increases fertility

We evaluated the effect on fertility of in vitro exposure of thawed frozen bull sperm to synthetic FertPlus(R) peptide prior to artificial inseminatio n (AI). The peptide represented a 60-amino acid sequence within rat prosapo sin. Commercial cryopreserved semen was from three Holstein bulls. Onset of estrus in groups of Holstein nulliparous heifers was synchronized via inje ction of prostaglandin F-2-alpha, and heifers were scheduled for Af 8-24 ho urs after estrus was detected. Semen was thawed, diluted to 2.4 x 10(6) spe rm/ml with buffer, and split to provide control and exposed aliquots (0 or 30 mu M peptide) that were incubated at 37 degrees C for 10 minutes and the n were held at 32 degrees C. The two aliquots of semen then were used on an alternate basis 2-65 minutes later to inseminate females. Each Al lane per female) involved the deposit of similar to 250,000 sperm into each uterine horn. This procedure for Al was used to reduce the pregnancy rate with con trol semen to below the maximum Value for a given bull and to facilitate de tection of any beneficial effect of the peptide. For each hull, similar to 32 heifers were inseminated with control semen, and similar to 32 heifers w ere inseminated with peptide-exposed semen. Pregnancy was evaluated ultraso nically similar to 60 days after At. After excluding one group of heifers w ith unusually low fertility. averaged across all animals, a 29% increase in pregnancy rate resulted from exposure of sperm to peptide (P < 0.04; one-t ailed chi-square test; means were 48 vs. 62%). Pregnancy rates for the thre e bulls for control and peptide-exposed semen, respectively, were 42 and 62 %, 44 and 64%, and 56 and 61%; means in the first two pairs of values tende d to differ (P similar or equal to 0.10). These observations should be conf irmed with sperm from other bulls used in a more conventional manner. Howev er, with insemination of a limiting number of cryopreserved sperm, brief ex posure of the thawed bull sperm to FertPlus(R) peptide appeared to improve fertility dramatically.