High-performance liquid chromatography with peroxyoxalate chemiluminescence determination of propentofylline concentrations in rat brain microdialysate
Y. Hamachi et al., High-performance liquid chromatography with peroxyoxalate chemiluminescence determination of propentofylline concentrations in rat brain microdialysate, J CHROMAT B, 724(1), 1999, pp. 189-194
A high-performance liquid chromatographic determination of a neuronal cell
protective compound, propentofylline [3-methyl-1-(5-oxohexyl)-7-propyl-7H-p
urine-2(3H),6(1H)-dione] was performed combining a microdialysis technique
with peroxyoxalate chemiluminescence (PO-CL) detection. The microdialysate
was subjected to a fluorescent derivatization of propentofylline with 4-(N,
N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H) without f
urther cleanup, because the membrane used in the microdialysis excluded hig
h-molecular-mass proteins. The proposed method showed a good linearity in t
he determination range of 0.031 to 1.25 ng/injection; y (mu V)=4234 x (ng)-
13.43, r=0.9993 (y=peak height and x=amount of propentofylline). The very l
ow determination limit of 0.031 ng/injection was ca. 200 times more sensiti
ve than that of HPLC-UV determination. The HPLC-PO-CL method was applied fo
r the first time to determine propentofylline concentration in the dialysat
e obtained from the rat hippocampus after a single oral administration (25
mg/kg). Propentofylline showed its maximum extracellular fluid (ECF) concen
tration of 125.1+/-15.1 ng/ml (mean+/-SD, n=3) at 0.33 h after administrati
on. (C) 1999 Elsevier Science BN. All rights reserved.