Three strains of a gram-positive catalase-negative, facultatively anaerobic
coccus-shaped organism originating from human clinical samples were charac
terized by phenotypic and molecular taxonomic methods. Sequencing of genes
encoding 16S rRNA showed that the strains are phylogenetically closely rela
ted (99.9 to 100% sequence similarity) and represent a new subline within t
he genus Facklamia. The unknown bacterium was readily distinguished from al
l currently described species of the genus Facklamia (viz., Facklamia homin
is, Facklamia ignava, and Facklamia sourekii) by biochemical tests and elec
trophoretic analysis of whole-cell proteins. Based on phylogenetic and phen
otypic evidence, it is proposed that the unknown bacterium be classified as
Facklamia languida sp. nov. The type strain off. languida is CCUG 37842.