Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complex

Authors
Githui, WA Wilson, SM Drobniewski, FA
Citation
Wa. Githui et al., Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complex, J CLIN MICR, 37(4), 1999, pp. 1224-1226
Citations number
21
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
4
Year of publication
1999
Pages
1224 - 1226
Database
ISI
SICI code
0095-1137(199904)37:4<1224:SOIDFA>2.0.ZU;2-V
Abstract
Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycob acterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with I S6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberc ulosis complex isolates.