Renewable silica sol-gel derived carbon composite based glucose biosensor

An amperometric mediated glucose biosensor has been developed based on a so l-gel derived carbon composite material. Glucose oxidase and the mediator v inylferrocene have been immobilised within the porous, rigid and organicall y modified silicate network in the composite material. The organic group in the silicate network controls the hydrophobicity of the electrode surface and thus limits the wettability of the electrode surface. Various important fabrication factors controlling the biosensor performance have been invest igated systematically. The glucose biosensor can be renewed easily in a rep roducible manner by a simple polishing step and it has a long operational l ifetime. Applicability of the biosensor has been demonstrated in real sampl es and the results obtained by this biosensor corroborate well with a class ical UV spectrophotometric technique. (C) 1999 Elsevier Science S.A. All ri ghts reserved.