Immunoenzymatic detection of the new proliferation associated protein p100by means of a cellular ELISA: specific detection of cells in cell cycle phases S, G2 and M

In vitro proliferation assays are widely used in biomedical research. We de scribe the immunoenzymatic (ELISA) detection of a recently described prolif eration associated protein (p100) by means of a new monoclonal mouse IgG1 a ntibody (Ki-S2). P100 is a 100 kDa nuclear protein that is specifically det ected during the cell cycle phases S, G2 and M. Comparative studies on lect in-stimulated leukocytes using H-3-thymidine labelling and Ki-67 antibodies revealed a statistically significant positive correlation. Since p100 is a bsent in GO and G1 cells, its detection permits the precise and specific me asurement of actual cell cycle events under culture conditions. (C) 1999 El sevier Science B.V. AU rights reserved.