VACCINATION WITH MONOCLONAL ANTIIDIOTYPIC ANTIBODY ON IMMUNOCOMPETENTMICE BEARING HUMAN OVARIAN-CARCINOMA

Objective To observe the effects of anti-idiotypic monoclonal antibody 6B11 against ovarian carcinoma SKOV3 on immunocompetent mice BCF1 bot h in vivo and in vitro, so as to investigate the possibility of using it as a rumor vaccine. Methods After immunization with 6B11 and normal mice (NM) IgG respectively on 2 groups of BCF1, blood, lymphocytes an d rumor tissues were taken every other day. Ab3 was tested from blood samples. Subpopulations of lymphocytes were examined by FCM. The lymph ocytes from immunized BCF1 were also cultured with SKOV3 to observe th e chemotaxis and killing effects. Serial tissue sections were taken fr om BCF1 after immunization by SRCA implantation of SKOV3. TIL and visi ble cancer cells were examined carefully and compared with those in th e controls. Results Ab3 rose quickly after immunization with 6B11 but lowered down gradually till the 9th week. The CD4+/ CD8+ ratio of BCF1 changed markedly after immunization with 6B11. The immunized lymphocy tes showed a beautiful chemotaxis with and killing effect on SKOV3. Du ring in vivo examinations, TIL were found significantly earlier in the immunized BCF1 than in the controls and reached the peak earlier in t he former (on the 6th day) than in the latter, while the viability of tumor cells was vice versa between the two groups. Conclusion 6B11 may be used as a vaccine for not only active immunization but also preven tion of ovarian carcinoma.