Dk. Lee et A. Ramamoorthy, Determination of the solid-state conformations of polyalanine using magic-angle spinning NMR spectroscopy, J PHYS CH B, 103(1), 1999, pp. 271-275
Conformations of the powder samples of poly-L-alanine with molecular weight
s of 356 Da (tetraalanine), 15 000 Da (PLA-200), and 23 600 Da (PLA-333) we
re characterized by C-13 cross-polarization magic-angle spinning (CPMAS) an
d H-1 combined rotation and multiple pulse (CRAMPS) solid-state NMR spectro
scopy. From the C-13 and H-1 isotropic chemical shift values, it is predict
ed that the main chain conformations of tetraalanine and PLA-200 are mainly
beta-sheet while the conformation of PLA-333 is mainly an alpha-helix. It
is unusual and interesting that a high molecular weight homopolypeptide, PL
A-200, has a beta-sheet conformation rather than an alpha-helix conformatio
n. The effect of dichloroacetic acid (DCA) solvent on the backbone conforma
tion of these peptides was also studied. It is inferred from solid-state NM
R results that conformations of tetraalanine and PLA-333 are similar before
and after crystallization from DCA. On the other hand, the backbone confor
mation of PLA-200 is 60% alpha-helix and 40% beta-sheet after crystallizati
on from the DCA solvent. Variable temperature studies are also reported.