Diagnostic polymerase chain reaction assay for species A and D of the Anopheles dirus (Diptera : Culicidae) species complex based on ribosomal DNA second internal transcribed spacer sequence

Authors
Xu, XC Xu, JN Qu, FY
Citation
Xc. Xu et al., Diagnostic polymerase chain reaction assay for species A and D of the Anopheles dirus (Diptera : Culicidae) species complex based on ribosomal DNA second internal transcribed spacer sequence, J AM MOSQ C, 14(4), 1998, pp. 385-389
Citations number
46
Categorie Soggetti
Entomology/Pest Control
Journal title
JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION
ISSN journal
8756971X → ACNP
Volume
14
Issue
4
Year of publication
1998
Pages
385 - 389
Database
ISI
SICI code
8756-971X(199812)14:4<385:DPCRAF>2.0.ZU;2-3
Abstract
A polymerase chain reaction assay based on differences in the internal tran scribed spacer regions of ribosomal DNA was developed for distinguishing 2 members of the Anopheles dirus sibling species complex. This assay distingu ished An. dirus species A from species D by producing diagnostic bands, 374 base pairs (bp) in length for species A and 663 bp in length for species D . Both laboratory colonies and field collections from Hainan and Yunnan pro vinces of China were identified with 100% accuracy.