Determination of anisidine value in thermally oxidized palm olein by Fourier transform infrared spectroscopy

Fourier transform infrared (FTIR) spectroscopy with transmission cell is de scribed to predict anisidine value of palm olein. The calibration set was p repared by mixing the thermally oxidized palm olein and the unoxidized palm olein with certain ratios (w/w) covering a wide range of anisidine values. A partial least square (PLS) regression technique was employed to construc t a calibration model. This model was further accomplished by a validation step. The standard error of prediction found was 0.51. The precision of thi s method was shown to be comparable to the accuracy of the American Oil Che mists' Society method used for measurement of anisidine value, with co-effi cient of determination (R-2) of 0.99. The study showed that mid-band FTIR s pectroscopy combined with a PLS calibration technique is a versatile, effic ient, and accurate technique for the estimation of anisidine value of palm olein within about 2 min with less than 2 mL of sample.