Utilization of synthetic carnosine as a food additive is limited by both re
gulatory and economic hurdles. Therefore, the potential of producing carnos
ine-containing antioxidant extracts from an underutilized skeletal muscle s
ource, mechanically separated pork (MSP), was investigated. Carnosine-conta
ining MSP extracts were capable of inhibiting lipid oxidation both in vitro
and in salted ground pork. Heating (60-80 degrees C) the MSP extract remov
ed iron and increased in vitro antioxidant activity. Isolation of a low mol
ecular weight fraction of the MSP extract by ultrafiltration was effective
at decreasing iron but did not substantially increase in vitro antioxidant
activity. Freeze dried extracts (untreated, 80 degrees C, ultrafiltration p
ermeate) were capable of inhibiting both TBARS and lipid peroxide formation
in ground, salted pork stored at -15 degrees C. While MSP extracts were ca
pable of inhibiting lipid oxidation both in vitro and in salted, ground por
k, their antioxidant activity was low suggesting that their use as a food a
dditive would be impractical. (C) 1999 Elsevier Science Ltd. All rights res
erved.