Detection of partial and complete acrosome reaction in human spermatozoa: which inducers and probes to use?

The acrosome reaction (AR), an essential step for achieving mammalian ferti lization, was recently introduced as a means of clinical evaluation of male fertility. However, most of the available techniques for acrosomal status assessment (except those employing electron microscopy) do not define wheth er the measurements represent partial or complete AR. We, therefore, perfor med a crossover investigation of the types of inducers and probes required for detecting partial or complete AR in human spermatozoa. The acrosomal st atus before and after stimulation with four AR inducers was evaluated after incubation for 3 h in capacitating conditions. We used a fluorescence-acti vated cell sorter with fluorescein isothiocyanate-conjugated monoclonal ant ibody CD46 (FITC-CD46) targeting the inner acrosomal membrane for detecting a complete AR, and fluorescein isothiocyanate-Pisum sativum agglutinin (FI TC-PSA) targeting the acrosomal content for detection of both partial and c omplete AR. Without stimulation or following stimulation with progesterone, follicular fluid (FF) or phorbol myristate ester (PMA), the AR could be de tected with FITC-PSA but not with FITC-CD46. Following stimulation with the calcium ionophore A23187, the AR could be detected by both FITC-PSA and FI TC-CD46. These results suggest that spontaneous AR as well as AR induced by progesterone, PMA and FF are partial. In contrast, the AR induced by A2318 7 is total, i.e. both partial and complete. These findings are valuable for both research and clinical purposes and are a step towards an internationa l agreement on a standard test for human sperm AR, for which there is an ur gent need.