Db. Hausman et al., Endocrine regulation of fetal adipose tissue metabolism in the pig: Interaction of porcine growth hormone and thyroxine, OBES RES, 7(1), 1999, pp. 76-82
Objective: This study tested the hypothesis that combined treatment of thyr
oxine (T-4) and growth hormone (GH) could normalize cellular and metabolic
aspects of adipose tissue development of hypophysectomized fetal pigs.
Research Methods and Procedures: On day 70 of gestation, pig fetuses were h
ypophysectomized by microcauterization or remained intact. Hypophysectomize
d fetuses remained untreated or were treated from day 90 to day 105 of gest
ation with T-4, GH, or a combination of both hormones.
Results: Body weights were unaffected by hypophysectomy or hormone treatmen
t. De novo lipogenesis in subcutaneous adipose tissue was increased 10-fold
by hypophysectomy, consistent with our previous results. This increase was
abolished by GH treatment in the hypophysectomized fetuses. In contrast, T
-4 treatment of the hypophysectomized fetuses resulted in a 12-fold further
increase in adipose tissue lipogenesis, an effect that was negated by conc
omitant administration of GH. Lipolytic response to isoproterenol was decre
ased by hypophysectomy, unaffected by GH treatment, and restored to intact
values by T-4 or by T-4+GH treatment in the hypophysectomized fetuses.
Discussion: In contrast to T-4, GH does not influence serum insulin-like gr
owth factor-I or adipose tissue lipolysis, but decreases lipogenesis in the
fetal pig. However, replacing both T-4 and GH normalized hypophysectomized
fetuses to a greater extent than either GH or T-4 alone. Thus, any influen
ce of thyroid hormones on stimulating adipose tissue lipogenesis in the dev
eloping fetal pig may be normally counterregulated by pituitary-derived gro
wth hormone.