Regional heterogeneity and intra-observer variability of DNA-content and cell proliferation markers determined by flow cytometry in head and neck tumours

We analyzed regional heterogeneity of DNA content and of proliferative capa city. DNA-diploid as well as DNA-non-diploid tumour cell populations both w ere found to be present in 13% of the cases. The average coefficient of var iation (CV) of DI appeared to be 2%. The CV's of G(1)PF, LI, and SPF, were 4%, 20%, and 25%, respectively. The CV's of T-s and for T-pot appeared to b e 17% and 30%, respectively. The ratio of the intratumoural variability to the intertumoural variability, was lowest for DI. Intraobserver variability was evaluated. The correlation coefficient (CC) for LI and DI was 0.99 and 0.98, respectively, and for relative movement (RM) was 0.71. We concluded that for DI of head and neck tumours both representativity and reproducibil ity were better when compared with those for SPF, LI, G(1)PF, T-s, and T-po t. (C) 1999 Elsevier Science Ltd. All rights reserved.