Nicotianamine chelates both Fe-III and Fe-II. Implications for metal transport in plants

Citation
N. Von Wiren et al., Nicotianamine chelates both Fe-III and Fe-II. Implications for metal transport in plants, PLANT PHYSL, 119(3), 1999, pp. 1107-1114
Citations number
34
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT PHYSIOLOGY
ISSN journal
00320889 → ACNP
Volume
119
Issue
3
Year of publication
1999
Pages
1107 - 1114
Database
ISI
SICI code
0032-0889(199903)119:3<1107:NCBFAF>2.0.ZU;2-D
Abstract
Nicotianamine (NA) occurs in all plants and chelates metal cations, includi ng Fe-II, but reportedly not Fe-III. However, a comparison of the Fe-II and Zn-II affinity constants of NA and various Fe-III chelating aminocarboxyla tes suggested that NA should chelate Fe-III High-voltage electrophoresis of the FeNA complex formed in the presence of Fe-III showed that the complex had a net charge of 0, consistent with the hexadentate chelation of Fe-III. Measurement of the affinity constant for Fe-III yielded a value of 10(20.6 ), which is greater than that for the association of NA with Fe-II (10(12.8 )). However, capillary electrophoresis showed that in the presence of Fe-II and Fe-III, NA preferentially chelates Fe-II, indicating that the Fe(II)NA complex is kinetically stable under aerobic conditions. Furthermore, Fe co mplexes of NA are relatively poor Fenton reagents, as measured by their abi lity to mediate H2O2-dependent oxidation of deoxyribose. This suggests that NA will have an important role in scavenging Fe and protecting the cell fr om oxidative damage. The pH dependence of metal ion chelation by NA and a t ypical phytosiderophore, 2'-deoxymugineic acid, indicated that although bot h have the ability to chelate Fe, when both are present, 2'-deoxymugineic a cid dominates the chelation process at acidic pH values, whereas NA dominat es at alkaline pH values. The consequences for the role of NA in the long-d istance transport of metals in the xylem and phloem are discussed.