Light-induced changes in hydrogen, calcium, potassium, and chloride ion fluxes and concentrations from the mesophyll and epidermal tissues of bean leaves. Understanding the Ionic basis of light-induced bioelectrogenesis
S. Shabala et I. Newman, Light-induced changes in hydrogen, calcium, potassium, and chloride ion fluxes and concentrations from the mesophyll and epidermal tissues of bean leaves. Understanding the Ionic basis of light-induced bioelectrogenesis, PLANT PHYSL, 119(3), 1999, pp. 1115-1124
Noninvasive, ion-selective vibrating microelectrodes were used to measure t
he kinetics of H+, Ca2+, K+, and Cl- fluxes and the changes in their concen
trations caused by illumination near the mesophyll and attached epidermis o
f bean (Vicia faba L.). These flux measurements were related to light-induc
ed changes in the plasma membrane potential. The influx of Ca2+ was the mai
n depolarizing agent in electrical responses to light in the mesophyll. Cha
nges in the net fluxes of H+, K+, and Cl- occurred only after a significant
delay of about 2 min, whereas light-stimulated influx of Ca2+ began within
the time resolution of our measurements (5 s). In the absence of H+ flux,
light caused an initial quick rise of external pH near the mesophyll and ep
idermal tissues. In the mesophyll this fast alkalinization was followed by
slower, oscillatory pH changes (5-15 min); in the epidermis the external pH
increased steadily and reached a plateau 3 min later. We explain the initi
al alkalinization of the medium as a result of CO2 uptake by photosynthesiz
ing tissue, whereas activation of the plasma membrane H+ pump occurred 1.5
to 2 min later. The epidermal layer seems to be a substantial barrier for i
on fluxes but not for CO2 diffusion into the leaf.