TISSULAR MICRODIALYSIS - PRACTICAL AND TH EORETICAL ASPECTS

Microdialysis is now widely used for the study of peripheral tissues. This technique allows the monitoring of metabolites and small molecule s from the extracellular compartment as well as local delivery of meta bolically active agents to this compartment. The purpose of this revie w was to present a practical approach (different types of probes, mole cular cut-off, perfusion rate, dialysis buffer) and discuss the theore tical aspects of in vivo microdialysis (probe efficiency, recovery, pe rfusion rate, quantification of tissular metabolism). Our goal was to provide re searchers with the practical tools needed for rapid mastery of in vivo tissular microdialysis.